40ml Manual Double Phase Blood Culture Bottle
CHL-M12
Anaerobic and AerobicAdult 5-10ml
Infant 1-5ml
INTENDED USE
Ready to use culture medium based assay for the qualitative detection of microorganisms in body fluids like blood, ascites, cerebrospinal fluid, etc, offering isolated strains to carry out susceptibility tests.
INSTRUCTION
Blood culture is one of the most important and critical procedures
performed in the microbiology laboratory. Since blood is normally sterile, the isolation and identification of an organism have great diagnostic significance.
Mostly used to diagnose microorganisms especially for some fastidious organisms such as Haemophilus influenza, Neisseria meningitides, streptococcus pneumonia, bacterium burgeri, actinobacillus, cardiobacterium hominis, kingella. It is important to diagnose infection bacteremia and septicemia.
The Biphase system is simple for separation cultivation, non-contaminate, easy to observe the isolated colony on the agar. The isolated colony can use for antibiotics susceptibility tests and strains identification.
Blood culture has a Biphase blood culture and Anaerobic blood culture two types.
Biphase blood culture: peptone, beef extract,hemin,yeast,SPS,autoclaving
Anaerobic blood culture: peptone,beef extract,hemin,yeast,SPS,cysteine hydrochloride,autoclaving
PRINCIPLE
The Biphase system is simple for separation cultivation, non-contaminate, easy to observe the isolated colony on the agar. The isolated colony can use for antibiotics susceptibility tests and strains identification.
STORAGE AND VALIDITY
Store all components at 4-25 ºC. Avoid strong light The validity duration is 18 months
KIT COMPONENTS
peptone,beef extract,hemin,yeast,SPS,cysteine hydrochloride,autoclaving
Blood or ascites, sterile operation, can inject the sample in the bottle directly, no need special operation.
PROCEDURE
1. Check if the bottle is contaminated or broken.
2. Prepare and label the appropriate blood culture bottle
3. Do not unscrew the cap. Remove the plastic top of the screw cap on the blood culture bottle.
4. Disinfect the visible part of the rubber plug with iodine or ethyl alcohol (70%) and allow drying.
5. Collect approximately 5-10 ml of adult patients' blood or 1-3 ml of infant patients' blood
6. Inject blood, ascites, or cerebrospinal fluid into the culture bottle, after being collected with aseptic operations. Then disinfect the rubber plug again and replace the plastic top.
7. After the inoculation, lean the bottle several times, and incubate it, erectly at 35-37 ºC for 24 hours.
INTERPRETATION OF RESULT
1. Report the positive results if turbidity in the culture broth, hemolysis of blood corpuscles, air bubbles, or colonies are observed. Carry out susceptibility tests and strain identification on positive samples.
2. Soak agar state into broth every day and keep on incubating the bottle erectly if those phenomena do not occur, until the 7th day.
3. If the abovementioned phenomena still do not occur after 7 days of culturing, report a negative result after confirming with microscopy and blind passage.
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