CE Marked PCR Ab Antibody Neutralization Whole Blood Rapid Detection Device Kit

CE marked PCR Ab Antibody neutralizing whole blood rapid test device kit  Sample requirement 1. Sample type: human serum or plasma. 2. Collect plasma with heparin sodium, heparin lithium, and EDTA-K2 anticoagulant. 3. Centrifuge to remove precipitate from the sample before testing. Before centrifugation, make sure that

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CE marked PCR Ab Antibody neutralizing whole blood rapid test device kit 
 
Sample requirement
1. Sample type: human serum or plasma.
2. Collect plasma with heparin sodium, heparin lithium, and EDTA-K2 anticoagulant.
3. Centrifuge to remove precipitate from the sample before testing. Before centrifugation, make sure that a
complete blood clot is formed in the serum sample. Some samples, especially blood samples taken from
patients who are resistant to anticoagulants or who are receiving thrombolytic therapy, have a longer clot
clotting time. If the sample is centrifuged before the clot is formed, the result may be incorrect due to the
presence of fibrinogen.
4. Samples contaminated with microorganisms cannot be used. Patient samples must be handled carefully,
and it is recommended to use disposable pipettes or pipette tips to avoid cross-contamination.
5. In order to achieve the best test results, it is recommended to observe the conditions of all samples before
testing on the machine. If there are obvious bubbles or fiber suspensions, try to remove them.
6. After removing blood clots or red blood cells, the sample can be stored for 7 days at 2-8ºC, and for 12
months at -20±5ºC. Avoid repeated freezing and thawing of samples no more than 3 times.
7. When transporting samples, packaging and labels should comply with national or international
regulations on the transportation of clinical samples and infectious substances. The samples should be
transported under refrigerated or frozen (dry ice) conditions. It is recommended to separate the samples
from blood clots or red blood cells before transport.
Testing Method
1. Washing solution preparation: Dilute the concentrated washing solution 25× with purified water at 1:25.
2. Sample dilution: Dilute the sample to be tested 1:10 with sample diluent, such as 10μL sample + 90μL
sample diluent, and mix thoroughly.
3. Positive control, Negative control: do not need dilution, use directly.
3. Adding samples: Add the Positive control, Negative control and diluted samples to the wells of the
Neutralizing Antibody Reaction Plate, 50μL per well, and set a blank well, then add 50μL RBD enzyme
marker.
4. Incubation: Cover the plate with a Seal Sheet and incubate at 37°C for 30 minutes.
5. Add ACE-2: After finishing the incubation, add 50μL of ACE-2 protein reagent without washing the
plate.
6. Incubation: Cover the plate with a Seal sheet and incubate at 37°C for 30 minutes.
7. Washing: Rinse 6 times with washing solution and pat to dry.
8. Color developing: Add 50μL of substrate A and B solution to each well, shake and mix, and develop
color at 37°C for 15 minutes avoiding light.9. Detection: Add 50μL Stop Solution to each well, shake and mix. Set the wavelength of the microplate
reader at 450nm (dual-wavelength detection is recommended, 630nm is used as the reference wavelength),
first use a blank hole to do zero calibration, and then measure the OD value of each hole.

CE Marked PCR Ab Antibody Neutralizing Whole Blood Rapid Test Device KitCE Marked PCR Ab Antibody Neutralizing Whole Blood Rapid Test Device KitCE Marked PCR Ab Antibody Neutralizing Whole Blood Rapid Test Device KitCE Marked PCR Ab Antibody Neutralizing Whole Blood Rapid Test Device Kit
 

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